Arginine as a General Acid Catalyst in Serine Recombinase …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790019/
WEBOct 4, 2013 · The C terminus, Gln-115, and Arg-54 are all distant from the active site, and the R54E mutation has no effect on reactions in the absence of regulatory sites. Henceforth, the His 6 -tagged R54E/Q115R construct will be referred to as “pseudo-WT,” and all additional mutations were made in this activated background.
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